ATP dependence of Na(+)-K+ pump of cold-sensitive and cold-tolerant mammalian red blood cells.
نویسندگان
چکیده
منابع مشابه
Demonstration of temperature dependence of Na(+)-K+ pump activity of human blood cells.
In our physiology laboratory course we introduced several simple but instructive experiments in which medical students make observations on their own blood cells. In this experiment, students measured and discussed the effect of different temperatures on Na+ and K+ distribution between blood cells and plasma. In venous blood of 35 female and 64 male students, plasma (extracellular) [Na+] and [K...
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These comments are directed toward the nature of the interaction between cardiotonic steroids, such as ouabain, and the red blood cell plasma membrane. I t has been known since 1953 (i) that one of the primary reactions associated with this class of steroids is their ability to inhibit the active transport of both Na and K in red cells as well as in a variety of other types of cells and tissues...
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External ATP causes a prompt increase in the Na and K permeability of dog red blood cells. By manipulating intra- and extracellular ion composition it is possible to observe ATP-induced net fluxes which can be explained in terms of the contribution of Na or K diffusion potentials to the membrane potential. Measurements of membrane voltage by a fluorescent dye technique confirm the existence of ...
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Ouabain-sensitive uncoupled Na+ efflux has been studied in human, pig, and rat red cells and in vesicles containing reconstituted kidney Na+/K+ pumps obtained from these same species. The red cells from the different species gave qualitatively similar results; the uncoupled Na+ efflux was 15-30% of the Na+/K+ exchange rate, and this flux was inhibited at 5 mM extracellular Na+ (Na+o). At higher...
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The interaction of extracellular Na (Na(o)), K (K(o)), and strophanthidin with the Na-K pump of the human red blood cell has been investigated. Inhibition by submaximal concentrations of strophanthidin rapidly reaches a level which does not increase further over a relatively long period of time. Under these circumstances, it is possible to apply a steady-state kinetic analysis to the interactio...
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ژورنال
عنوان ژورنال: The Journal of Physiology
سال: 1992
ISSN: 0022-3751
DOI: 10.1113/jphysiol.1992.sp019354